1.在项目根目录创建my_app.spec文件，内容如下：

# -*- mode: python ; coding: utf-8 -*-


block_cipher = None


a = Analysis(
    ['server.py'], # flask入口
    pathex=[],
    binaries=[],    
    datas=[("E:/**/templates","/templates"),("E:/**/static","/static")],# 将资源的绝对路径转换为你打包的exe的相对路径
    hiddenimports=[],
    hookspath=[],
    hooksconfig={},
    runtime_hooks=[],
    excludes=[],
    win_no_prefer_redirects=False,
    win_private_assemblies=False,
    cipher=block_cipher,
    noarchive=False,
)
pyz = PYZ(a.pure, a.zipped_data, cipher=block_cipher)

exe = EXE(
    pyz,
    a.scripts,
    a.binaries,
    a.zipfiles,
    a.datas,
    [],
    name='my_flask_app', #名字自己定
    debug=False,
    bootloader_ignore_signals=False,
    strip=False,
    upx=True,
    upx_exclude=[],
    runtime_tmpdir=None,
    console=True,
    disable_windowed_traceback=False,
    argv_emulation=False,
    target_arch=None,
    codesign_identity=None,
    entitlements_file=None,
    icon=['server.ico'], # 程序图标
)

2.开始打包

运行以下命令来使用PyInstaller根据my_app.spec文件打包flask应用程序：

pyinstaller my_app.spec

3.在dist目录添加资源文件